Case Number 102037 - Method for Determining Dietary Fat Absorption
Contact: Kellen Sensor
Description: The current invention is a composition used as a test meal comprising a predetermined amount of dietary fat and a predetermined amount of a non-absorbable fat marker, such as a sucrose polyester in the form of sucrose behenate. The method for measuring total dietary fat absorption by the digestive tract of a subject comprises the steps of administering ingestion of the test meal by the subject, collecting a sample of fecal matter at an interval following ingestion of the test meal, measuring the amount of the of non-absorbable fat marker recovered in the fecal sample and calculating the amount of dietary fat recovered from the test meal to determine the amount of dietary fat that was absorbed by the digestive tract of the subject. The methods of the invention can be used to diagnose malabsorption of dietary fat by the digestive tract of a subject or impairment of dietary fat digestion. The advantage of this current invention over existing tests is that patients may take only one test and only a single “marked” stool sample is required for analysis to determine the ratio of fat to marker in the stool and by comparison to the same ratio in the test meal, calculate the fraction of fat absorbed from the test meal. Sucrose behenate is an excellent marker for this purpose because it is currently consumed in food, is completely excreted, is handled by the G.I. tract as are normal dietary fats and is readily measurable by gas chromatography. Thus, this method provides a rapid and easy assessment of dietary fat absorption and avoids the cumbersome and laborious process currently in use. Investigators are currently nearing the completion of a study to compare the method with the quantitative fecal fat measurement in cystic fibrosis and have completed studies evaluating the sucrose behenate method against the quantitative fecal fat method in overweight adults taking Orlistat.
For more information see the Cincinnati Children's Hospital's List of Available Diagnostic Technologies and more specificially see here