Case Number 103023 - Osteoblast Targeted Reporter Mice
Contact: Lynn Briggs
Description: Dr. Thomas Clemens, while a Professor in the Department of Internal Medicine at the University of Cincinnati, developed a new mouse model - the Osteocalcin Cre transgenic mouse. The noninvasive monitoring of gene expression in vivo represents a novel methodology to examine individual genes in different tissue environments. The recent development of the cooled charged coupled device (CCCD) camera has enabled visualization of low quantities of photons emitted by internal mammalian tissue both in vitro and in vivo. To take advantage of this technique, Dr. Clemens created mice expressing the luciferase gene under the control of a segment of the human osteocalcin promoter. These mice express the luciferase gene only in mature osteoblasts and osteocytes. This mouse model has enabled the examination of bone regeneration to track bone specific gene expression following fracture and regeneration.
Dr. Clemens’ mouse was the first transgenic mouse model developed that expresses the human osteocalcin promoter driving luciferase.
A detailed description of this mouse can be seen in an article from the December 2003 issue of Journal of Bone and Mineral Research (JBMR); which you can access by clicking on the image below